KINETIKA HIDROLISIS PATI UBI JALAR KUNING MENJADI SIRUP GLUKOSA SECARA ENZIMATIS

Piasasi Cania, NIM. 122017019 (2021) KINETIKA HIDROLISIS PATI UBI JALAR KUNING MENJADI SIRUP GLUKOSA SECARA ENZIMATIS. Skripsi thesis, Universitas Muhammadiyah Palembang.

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Abstract

Abstrak Berdasarkan hasil Analisa Spektrofotometer Uv-vis bahwa pati ubi jalar kuning mengandung pati 88,1%. Polisakarida dapat dihidrolisis dengan enzim menjadi sirup glukosa. Kualitas sirup glukosa dapat diketahui dari kadar komposisi meliputi (Kadar glukosa, pati bereaksi, pati sisa, dan konversi pati). Penelitian kinetika reaksi hidrolisis pati ubi jalar kuning menjadi sirup glukosa ini adalah Untuk mengetahui kualitas sirup glukosa secara hidrolisis dengan menggunakan enzim pada pembuatan sirup glukosa dari pati ubi jalar kuning (Ipomea Batatas , L.) dan untuk mendapatkan data kinetika reaksi hidrolisis pati ubi jalar kuning menjadi sirup glukosa. Tanaman ubi jalar merupakan tanaman yang perawatannya tergolong mudah, tahan terhadap kekeringan, biaya perawatannya murah, tidak heran jika ubi jalar disukai banyak petani, terutama diusahakan sebagai tanaman palawija, sebagai tanaman gilir setelah padi. Impor sirup glukosa tiap tahun diperkirakan akan meningkat dengan rata-rata pertumbuhan 30%. Pada tahap liquifikasi ditambahkan 0,02 gram enzim α-amilase dan dipanaskan selama 40 menit pada suhu sekitar 80-110°C. setelah itu tahap sakarifikasi, sampel diatur suhunya menjadi 60°C kemudian ditambahkan 0,07 ml enzim glukoamilase dan dihidrolisis selama 7 hari. Di analisa kadar air, kadar gula BM rendah, kadar glukosa serta kinetika reaksinya. Sehingga didapatkan hasil terbaik sirup glukosa adalah pada waktu 168 jam dan volume aquadest 100 ml yaitu sebesar 66,8% memiliki nilai pati bereaksi 75,8%, pati sisa 12,3%, dan konversi pati sebesar 86%. Dan nilai konstanta kinetika reaksinya 0,0226/jam. Kata Kunci :sirup glukosa, hidrolisis, konstanta kinetika reaski, dan enzim Abstract Based on the results of the Uv-vis spectrophotometer analysis that yellow sweet potato starch contains 88.1% starch. Polysaccharides can be hydrolyzed by enzymes into glucose syrup. The quality of glucose syrup can be seen from the composition content (glucose content, reaction rate, residue, and starch conversion). The research on the kinetics of the hydrolysis reaction of yellow sweet potato starch into glucose syrup is to determine the quality of glucose by hydrolysis using glucose syrup from yellow sweet potato starch (Ipomea Batatas, L.) and to obtain data on the hydrolysis reaction of yellow sweet potato starch into glucose syrup. . Sweet potato plants are plants that are relatively easy to care for, resistant to drought, low maintenance costs, it is not surprising that sweet potatoes are favored by many farmers, especially planted as secondary crops, as a rotating crop after rice. Imports of glucose syrup annually are expected to increase with an average growth of 30%. In the liquefaction stage, 0.02 grams of -amylase enzyme were added and waited for 40 minutes at a temperature of around 80-110 °C. After the saccharification stage, the temperature was adjusted to 60°C, then 0.07 ml of glucoamylase was added and hydrolyzed for 7 days. In the analysis of air content, low BM sugar content, glucose levels and reaction kinetics. So that the best results obtained glucose syrup is at 168 hours and aquadest volume of 100 ml that is equal to 66.8% has a reacted starch value of 75.8%, remaining starch 12.3%, and starch conversion of 86%. And the value of the reaction kinetic constant is 0.0226/hour. Keywords: glucose syrup, hydrolysis, reaction kinetics constant, and enzymes

Item Type: Thesis (Skripsi)
Additional Information: Pembimbing : 1. Ir. Robiah, M.T 2. Ir. Ummi Kalsum, M.T
Uncontrolled Keywords: sirup glukosa, hidrolisis, konstanta kinetika reaski, dan enzim
Subjects: Kimia > Bahan Kimia Organis
Kimia > Teknologi Makanan
Kimia > Teknologi Produk-produk Organis
Divisions: Fakultas Teknik > Teknik Kimia (S1)
Depositing User: Mahasiswa Fakultas Teknik
Date Deposited: 13 Sep 2021 02:55
Last Modified: 13 Sep 2021 02:55
URI: http://repository.um-palembang.ac.id/id/eprint/17895

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